I Just Want to Be a Quiet Top Student - v2 Chapter 708

Han Meng began to edit the rat’s SHANK3 gene. At this time, the rat’s nucleotide daughter strands were not yet methylated, and the complementary parent strands contained methylated groups.

These methylations are non-mutated and have the same pairing properties as normal nucleotides in rats.

Since there is a time lag between DNA replication and the methylation of the daughter strands, this gave Han Meng an opportunity to scan for mismatched bases.

In an in vitro cell-free system, DNA is used as a template to imitate genes that control transcription in the organism. Such an operation is very expensive.

Expensive precision instruments are the fixed assets of the laboratory, and these fixed assets are depreciating over time. In addition to the consumption of various raw materials, consumables, and water and electricity, five experimenters will spend tens of thousands of dollars for a day of experimentation, regardless of whether there are results.

As the experiment progresses, the money will burn more and more.

The Central Biological Laboratory’s scientific research budget this year is 300 million yuan. The first quarter has just ended and it has spent more than 100 million yuan.

Burning money may not produce results. The four major pits in the biochemical material ring may be the meaning.

The five experimenters were stupidly unclear about the scientific research funding provided by the state and the sponsorship of Shen Qi outside. What they all knew was that the level of money burned by this laboratory was at an international level.

Huahan Gene’s laboratory burns more money, and its burn level is N times that of Shen Qi’s laboratory.

But the laboratory in the university cannot be compared with the companies outside. This is incomparable.

For biological research, if there is no sufficient funds, researchers will not be able to realize their academic ideals and life dreams.

One of the most important tasks of a laboratory owner is to make money.

The five experimenters can realize that Director Shen is not easy, and Director Shen is under pressure. In order to make more money to maintain the operation of the biological laboratory, Director Shen ran around and worked tirelessly.

The Central Physics Laboratory has achieved break-even, and the value of the patents they created has recovered the input cost, and even has a small surplus.

Chemical laboratories mainly research chemical drugs. They have a long time to recover costs, and they are now at the stage of struggle where input exceeds output.

What the five biological dogs in the center can do is to come up with some valuable research results that are widely recognized by the biological community and can create wealth as soon as possible.

Han Meng’s desire to produce results is the strongest. He calmly and meticulously operates the instrument to observe the progress of the experiment at the molecular level.

“MutS recognizes mismatched bases, and MutH recognizes two different single strands by combining with the unmethylated 5′-GATC-3′ sequence… The role of MutL is not obvious, it should be to coordinate the other two proteins. The activity of MutH, the deviation caused by the binding of MutH near the mismatch site recognized by MutS?”

The process of Han Meng working in the laboratory is also a process of constant thinking. There are many difficulties and puzzles in the research of rat IQ genes.

Solving difficult points and solving puzzles requires experimenters to sum up experience from a large amount of experimental data and keenly discover the key key.

If one road fails, try another one.

The same method of mismatch editing was adopted, but Han Meng’s editing path was adjusted. He controlled MutH to cut the 5’phosphate bond of the G base connected to the methylation sequence, and the DNA helicase then untwisted the double strand, and then the exposed single strand was degraded by the exonuclease until it reached the mismatch position. Point downstream.

The other four brothers tried different experimental routes with different editing methods, supplemented by computer analysis, and each came up with a set of preliminary plans that they thought was feasible.

Whether it is really effective, you need to invite rats to play.

Rats are white rats, and the team raised by Shen Qi are Wistar rats, which are most widely used in animal experiments.

The breeding room is next to the biological laboratory, where there is constant temperature and humidity, food and drink, and the living conditions and quality of life of the rats are maintained at a high level.

Wistar female rats are very fierce during lactation, and they often actively bite the hand of the staff who reach into the cage during feeding.

Under normal circumstances, these rats are relatively docile and can be raised in large numbers in a cage.

Han Meng and other five experimenters entered the breeding room. The rats of the smart group were about to move, rubbing their front paws off the ground, seeming to be pleased.

The rats in the normal group murmured and rolled their small eyes, their small noses moved and sniffed something, they felt the arrival of non-like creatures.

The rats of the idiot group huddled together in a daze, indifferent.

Everything has spirituality. People who study animals must first understand animals.

Cui Hualin observed the rats in the normal group in front of their cages. He suggested: “Before gene-editing them, please have a good meal.”

“It should be.” Han Meng nodded, and arranged for the rats to eat well.

There are national standards for the feed for experimental rats, and these rats usually eat the white rat feed that meets the national standard.

Although the white rat feed is rich in nutrients, it has fewer choices of flavors.

The favorites of these rats are fresh cabbage, cucumber, apple, sunflower seeds, blackcurrant, cherry seeds and other fruits and vegetables.

Each of the five experimenters selected a common group of rats in a cage, and asked their friends to eat fresh fruits and vegetables.

The rats in the normal group who ate the food were very happy. They beat back and forth, and laughed heartily.

Although they all sound like squeaks, if you carefully identify them, you will find that the squeaks with different frequencies represent different moods of the rats.

“Eat, eat more.” Cui Hualin continued to feed his boys fresh fruits and vegetables. He has been studying and working in biology for so many years, and he has suffered more than one rat life.

The other four brothers have more lives in the hands of rats. They have done a lot of experiments with white rats. They respect these small animals and they respect life. If they have to kill the experimental mice, they try to choose the least painful treatment method.

Compared with the white mice that undertake drug experiments, bacteria experiments, virus experiments, radioactivity experiments, and pathology experiments, these white mice used for IQ gene experiments will hardly feel pain in their short lifetime.

“Have you eaten?” Han Meng asked the little friend in the cage.

The friends replied: “Squeak!”

“Then let’s start the experiment?”

“squeak!”

“Thank you for your understanding, thank you for your cooperation.”

The five experimenters took their little friends out of the cage and moved to the next biological laboratory.

The rats were first anesthetized on the experimental table, and then the experimenters operated the gene editing equipment. UU reading www.uukanshu. com performs SHANK3 gene editing on rats.

Han Meng performed biological experiments on the experimental mice he selected in accordance with the experimental protocol he obtained in the in vitro cell-free system.

“Recognize the target base…”

“Cut the non-methylated chain…”

“Correct mismatched bases…”

The experiment went well.

The experimental mice were subjected to SHANK3 gene editing operation by the experimenters while they were sleeping.

“I’m done.”

“I’m done too.”

“Next, let us look forward to a miracle together.”

I just want to be a quiet schoolmaster

I just want to be a quiet schoolmaster